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plenticrispr v2 backbone  (Addgene inc)


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    Addgene inc plenticrispr v2 backbone
    Plenticrispr V2 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenticrispr v2 backbone/product/Addgene inc
    Average 93 stars, based on 18 article reviews
    plenticrispr v2 backbone - by Bioz Stars, 2026-06
    93/100 stars

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    Journal: iScience

    Article Title: Predicting transcription factor activity using prior biological information

    doi: 10.1016/j.isci.2024.109124

    Figure Lengend Snippet:

    Article Snippet: Two FOXO1 CRISPR guide RNAs in a pLentiCRISPR v2 backbone were obtained from GenScript.

    Techniques: Software, CRISPR, Recombinant, Sequencing

    FOXO1 is a critical mediator of response to venetoclax in AML (A) Priori scores generated from RNA-seq of 859 patients with AML. Spearman correlation of Priori scores and ex vivo drug response AUC data. (B and C) Spearman correlation of ranked venetoclax AUC and ranked (B) FOXO1 Priori scores or (C) FOXO1 normalized counts. Statistical significance was determined using the Spearman correlation p value with an FDR post-test correction. (D and E) THP-1 cells were transduced with lentiviral particles harboring expression cassettes for hSpCas9 and a non-targeting or FOXO1 guide RNA. Cells were cultured for 3 days along a 7-point curve with venetoclax. Cell viability was assessed by CellTiter Aqueous colorimetric assay. ns = not significant; ∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.001, ∗∗∗∗ = p < 0.0001.

    Journal: iScience

    Article Title: Predicting transcription factor activity using prior biological information

    doi: 10.1016/j.isci.2024.109124

    Figure Lengend Snippet: FOXO1 is a critical mediator of response to venetoclax in AML (A) Priori scores generated from RNA-seq of 859 patients with AML. Spearman correlation of Priori scores and ex vivo drug response AUC data. (B and C) Spearman correlation of ranked venetoclax AUC and ranked (B) FOXO1 Priori scores or (C) FOXO1 normalized counts. Statistical significance was determined using the Spearman correlation p value with an FDR post-test correction. (D and E) THP-1 cells were transduced with lentiviral particles harboring expression cassettes for hSpCas9 and a non-targeting or FOXO1 guide RNA. Cells were cultured for 3 days along a 7-point curve with venetoclax. Cell viability was assessed by CellTiter Aqueous colorimetric assay. ns = not significant; ∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.001, ∗∗∗∗ = p < 0.0001.

    Article Snippet: FOXO1 gRNA in pLentiCRISPR v2 backbone (#1) , Genscript , Sequence: GCTCGTCCCGCCGCAACGCG.

    Techniques: Generated, RNA Sequencing, Ex Vivo, Transduction, Expressing, Cell Culture, Colorimetric Assay

    Journal: iScience

    Article Title: Predicting transcription factor activity using prior biological information

    doi: 10.1016/j.isci.2024.109124

    Figure Lengend Snippet:

    Article Snippet: FOXO1 gRNA in pLentiCRISPR v2 backbone (#1) , Genscript , Sequence: GCTCGTCCCGCCGCAACGCG.

    Techniques: Software, CRISPR, Recombinant, Sequencing